Non-Trypfophan Fluorescence of Crysfallins From Normal and Cotoroctous Human Lenses

نویسندگان

  • George J. H. Bessems
  • Eric Keizer
چکیده

The soluble proteins of a newborn human lens exhibit almost no non-tryptophan fluorescence. On aging, an increase in the fluorescence of all native crystallins is found except for 72-crystallin. With formation of nuclear cataract, a further increase is seen for 7,-crystallin. The fluorophore, excitation 355 nm/emission 420 nm, is mainly associated with one species of the Yi-crystallin population. It is also present as such in the soluble fraction and increases significantly with nuclear cataract formation. At least one of the 7,-crystallins seems to play an important role in the cataractogenic process. Invest Ophthalmol Vis Sci 28:1157-1163, 1987

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تاریخ انتشار 2005